Page 39 - The autoimmune hypothesis of narcolepsy and its unexplored clinical features M.S. Schinkelshoek

Supplementary material
Supplementary table 2.1. Differences in distribution of HLA alleles in trans with HLA-DQB1*06:02 between NT1 patients with symptom onset in childhood after the H1N1 pandemic and the other included NT1 patients. P-values are derived from Pearson Chi-Square tests. Pc-values are calculated using the Šidák-Holm correction for multiple comparisons based on k = 13.
HLA associations after 2009 H1N1 pandemic
N
DQ2
DQ4
DQ7
DQ8
DQ9 DQB1*05:01 DQB1*05:02 DQB1*05:03 DQB1*06:01 DQB1*06:02 DQB1*06:03 DQB1*06:04 DQB1*06:09
Post-H1N1 and symptom onset in childhood
66
8 (11.9%) 1 (1.5%) 22 (33.3%) 9 (13.4%) 1 (1.5%)
1 (1.5%)
2 (3.0%)
1 (1.5%)
0 (0%)
13 (19.7%) 1 (1.5%)
7 (10.4%) 0 (0 %)
Other NT1 OR patients
459 NA
74 (16.1%) 0.71 23 (5.0%) 0.29 108 (23.5%) 1.6 47 (10.2%) 1.1 20 (4.3%) 0.33 34 (7.4%) 0.19 20 (4.4%) 0.68 17 (3.7%) 0.40 2 (0.4%) NA 72 (15.7%) 1.3 5 (1.1%) 1.4 34 (7.4%) 1.5 3 (0.7%) NA
95% CI
NA
0.32-1.5 0.04-2.2 0.93-2.8 0.60-2.1 0.04-2.5 0.03-1.4 0.16-3.0 0.05-3.1 NA 0.67-2.5 0.16-12.0 0.62-3.4 NA
p-value pc-value
NA NA
0.382 0.998 0.198 0.943 0.084 0.682 0.425 0.999 0.264 0.981 0.070 0.611 0.602 1.000 0.361 0.997 0.589 1.000 0.435 0.999 0.770 1.000 0.383 0.998 0.507 1.000
H1N1 = 2009 pandemic (H1N1)pdm09 influenza A strain; HLA-DQ2/4/7/8/9 = human leucocyte antigen molecule encoded by the HLA-DQB1*02:01/- DQB1*04:01/-DQB1*03:01 or -03:04/- DQB1*03:02 or -03:05/- DQB1*03:03 allele; NA = not applicable; NT1 = narcolepsy type 1; OR = Mantel-Haenszel common odds ratio; pc-value = corrected p-value.
Supplementary table 2.2. Distribution of HLA-DQB1 alleles in trans with HLA-DQB1*06:02 of narcolepsy type 1 patients diagnosed in 2010 and 2011.
N
DQ2
DQ4
DQ7
DQ8
DQ9 DQB1*05:01 DQB1*05:02 DQB1*05:03 DQB1*06:01 DQB1*06:02 DQB1*06:03 DQB1*06:04 DQB1*06:09
NT1 diagnosis in 2010-2011
47
9 (19.1%) 2 (4.3%) 12 (25.5%) 6 (12.8%) 0 (0%)
5 (10.6%) 0 (0%)
2 (4.3%) 0 (0%)
7 (14.9%) 1 (2.1%) 3 (6.4%) 0 (0%)
Adults Children
21 26
HLA-DQ2/4/7/8/9 = human leucocyte antigen molecule encoded by the HLA-DQB1*02:01/- DQB1*04:01/-DQB1*03:01 or -03:04/-DQB1*03:02 or -03:05/-DQB1*03:03 allele; NT1 = narcolepsy type 1.
3 (14.3%) 1 (4.8%) 5 (23.8%) 1 (4.8%) 0 (0%)
4 (19.0%) 0 (0%)
2 (9.5%) 0 (0%)
4 (19.0%) 0 (0%)
1 (4.8%) 0 (0.0%)
6 (23.1%) 1 (3.8%) 7 (26.9%) 5 (19.2%) 0 (0%)
1 (3.8%) 0 (0%)
0 (0%)
0 (0%)
3 (11.5%) 1 (3.8%) 2 (7.7%) 0 (0.0%)
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