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MIR147B: A NOVEL KEY REGULATOR OF IL-1β-MEDIATED INFLAMMATION IN ASTROCYTES
Figure 3. Proliferation of astrocytes after transfection with miR146a and miR147b mimics. Fetal human astrocytes were transfected for 24 hours with miRNA mimic (50 nM) and after a total of three days stained with anti-Ki67 antibody (A and B) or propidium iodine (PI, C). A Representative Ki67 staining in control cells and cells treated with miR146a or miR147b mimic. B Quantification of Ki67 staining showed decreased proliferation after miR146a and miR147b mimic transfection compared to control. C Both miR146a and miR147b mimic transfected cells showed lesser cells in the S-phase compared to control cells. Data are expressed relative to the levels observed in cells treated with transfection reagent only and are mean ± SEM of two experiments from two donors performed in triplicate. Cells in A were counterstained with phalloidin (actin filaments; red) and diamidino-2-phenylindole, DAPI (nuclei; blue). Scale bar in A: 50 μM. *p<0.05, **p<0.01 compared to control, Mann Whitney U test.
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