Page 75 - Magnesium-based supports for stem cell therapy of vascular disease - Mónica Echeverry Rendón

IMPROVEMENT OF CORROSION RESISTANCE OF COMMERCIAL PURE MAGNESIUM AFTER ITS MODIFICATION BY SINGLE AND TWO-STEP ANODIZATION
Fig.9. Cell proliferation of fibroblast growing on Mg samples after 24 and 72 h.
Cell-material interaction showed differences basically between samples with and without treatment. For the an- odized samples in general, a typical morphology of the osteoblast was observed after the actin staining (Fig.12). Healthy cells following the configuration of the coatings and the starting of the monolayer formation process were observed. Oppositely, cells growing in c.p Mg were unhealthy and actin was non-labelled and fragmented nucleus were observed, indicating a process on cell died on the surfaces of the material (Fig.12-F).
Fig.10. Cell-material interaction of osteoblast in surfaces of Mg. Cytoskeleton of osteoblasts were stained with phalloidin (red) and nuclei with DAPI (blue). Viable cells (red) were observed on samples NAF(A), HMT(B), MAN(C), NAF-HMT (D) and NAF¬MAN (E), except for c. p Mg where only occasional frag- mented nuclei were observed (F)
Irrespective of anodization all samples showed a high hemocompatiblity because no hemolysis occurred (Fig. 13A) while no thrombin generation occurred (Fig. 13 B, C) except for c.p Mg.
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