Page 14 - Magnesium-based supports for stem cell therapy of vascular disease - Mónica Echeverry Rendón

CHAPTER 1
tion. Because Mg is a highly reactive material to different environments such as chemicals or aqueous solutions, it is important to validate that the integrity of the coatings remains intact. This is relevant because the material will be implanted in vivo in the future which requires adequate sterilization.
Besides the material’s perspective, a biological characterization of surface-modified Mg on different cell types relat- ed to (repair of) cardiovascular tissue was performed. In Chapter 6 the cytotoxic effect of surface-modified c.p Mg on fibroblasts, endothelial cells, smooth muscle cells, adipose tissue-derived stromal cells (ASC) and macrophages was investigated. The biological function of ASCs in direct and indirect evaluations was investigated in more detail. Assessment of proliferation, apoptosis, angiogenesis and wound healing revealed the protective effect of coatings of Mg on the survival and functionality of ASCs. In addition, different models or experimental approaches for the evaluation of Mg in vitro and ex-vivo were studied and are described in Chapter 7. There, the effect of changes in pH and Mg2+ concentration was evaluated in HUVECs and SMCs. Moreover, functional experiment to verify the effect of corrosion products from Mg on HUVECs were performed.
This thesis concludes with a General Discussion and Perspectives Chapter (8) in which we compare and validate our overall results and generate new dogmas and directions for future research.
It is important to emphasize that our investigations were the first approach to a new concept, therefore the questions that were raised outnumber the answers obtained. Therefore, our studies are an important basis of study for future work on the application of surface-modified and cell-loaded c.p Mg for therapeutic purposes that extend beyond cardiovascular disease
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