Page 170 - Breeding and regulatory opportunities, Renaud

Chapter 5

Table 5.2 Trait means1 of phytochemicals of 23 broccoli cultivars grown across four pair combinations

of location (Maine/Oregon), season (Fall/Spring) two-years combined and management system
(Conventional/Organic), 2006-2008.

Maine
Oregon

Fall Spring
Fall
Spring

2006-2007 2007-2008 2006-2007 2007-2008
Mean
Mean
Combined Combined
Combined
Combined

COCO COCO

Glucoraphanin 5.31 e 3.77 bc 3.56 b 4.06 c 4.18 3.46 b 3.03 a 4.64 d 4.51 d 3.91

Glucobrassicin 1.06 b 0.90 a 1.45 c 1.33 c 1.19 5.14 f 5.51 g 2.24 d 2.70 e 3.90

Neoglucobrassicin 0.46 a 0.40 a 2.16 c 1.85 b 1.22 2.34 c 3.20 d 4.32 e 5.10 f 3.74

δ-Tocopherol 2.34 c 2.77 d 1.91 b 1.70 a 2.18 3.53 e 3.66 e 1.91 b 2.24 c 2.83

γ-Tocopherol 4.67 c 4.40 c 2.63 a 2.98
b 3.67
8.48 d 8.73 d 3.31 b 3.22 b 5.94

α-Tocopherol 25.83 a 27.33 a 38.61 b 40.51 bc 33.07 43.04 c 43.20 c 40.52 bc 42.25 c 42.25

Lutein 11.49 a 12.47 a 15.53 b 15.93 b 13.85 15.91 b 16.04 b 16.48 b 17.81 c 16.56

Zeaxanthin 0.81 a 0.83 ab 0.87 ab 0.88 b 0.85 0.83 ab 0.84 ab 1.02 c 1.02 c 0.93

β-Carotene
12.98 a 13.25 a 28.73 c 29.71 c 21.16 29.10 c 30.10 c 25.16 b 25.80 b 27.54

1 Values in the table are means. Means of the same letter in the same row are not signiicantly diferent
at the P < 0.05 level.

5.3.2 Partitioning of variance components

Glucosinolates

For glucoraphanin across all trials in both regions, Genotype (G) main efect

accounted for the largest proportion of variance, followed by G×L×S interaction

(Table 5.3). There was no Management (M) main efect, but M contributed

to the three (L×S×M and G×S×M) and four-way interactions (G×L×S×M). In

contrast to glucoraphanin, Location (L) had the largest efect for glucobrassicin

and neoglucobrassicin across all trials in both regions, followed by the L×S

interactions. For neoglucobrassicin the S and G main efect was more important

than for glucobrassicin. When trials were further partitioned by location, a G

and S main efect was apparent for neoglucobrassicin in both locations; for

glucobrassicin the S main efects was only apparent in Oregon and not in Maine

(Supplemental Table 5.2ab). There was M main efect for glucobrassicin and

neoglucobrassicin, but not for glucoraphanin, and no G×M interaction for all

glucosinolates.

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