Page 86 - Physico-Chemical Niche Conditions for Bone Cells

PFF affects pre-osteoblast behavior
visualized clearly at the stress fiber termini of the cells treated by mechanical loading [13]. Therefore, our first aim was to map any rapid (seconds, minutes) changes of apex height and fluid dynamics in an osteoblast after a single bout of a precisely defined load.
In this study, our aim was two-fold: 1) To map any rapid (seconds, minutes) changes of apex height and fluid dynamics over an osteoblast after a single bout of mechanical loading, and 2) To investigate whether osteoblasts produce (a mix of) signaling factors in the intermediate-term (hours) that affect osteogenic differentiation in the long-term (days). We showed images of a live cell during mechanical loading (immediate impact), and quantified immediate fluid dynamics over the cell by FE modeling. We tested whether a single bout of mechanical stimulation by PFF modulates the distribution of fluid dynamics over pre- osteoblasts (immediate impact), signaling molecule (nitric oxide (NO)) production (initial down- stream impact), metabolic activity, cell morphology, orientation, and expression of osteogenic genes, growth factors, and angiogenesis-related genes (short-term down-stream impact), as well as alkaline phosphatase (ALP) protein, collagen production and mineralization (long-term down-stream impact). Moreover, biochemical factors released during varying post-incubation times will have differential long-term impact on mineralization.
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