Page 62 - Physico-Chemical Niche Conditions for Bone Cells
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Shear stress modulates osteoblast cell and nucleus shape
RESULTS
Figure 1. Effect of 1 h pulsating fluid flow (PFF) on live and fixed cell and nucleus volumes of MC3T3- E1 osteoblasts. (A) Volumetric reconstruction and cell volume of live cells exposed to PFF at different time points. n = 24 cells from six glass slides. (B) Volumetric reconstruction and volume of cells and nuclei in formaldehyde-fixed cells subjected to 1 h static control culture or PFF treatment. n = 70 cells from 9 glass slides. CON, control; PFF, pulsating fluid flow. Significant effect of PFF, ** p < 0.01 and *** p < 0.001.
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