Page 119 - Physico-Chemical Niche Conditions for Bone Cells

Figure 1. Schematic of possible connection of mitochondria and cytoskeleton in a bone cell. (A) Mitochondria and actin filaments. (B) Mitochondria and microtubules. (C) Mitochondria and intermediate filaments.
and positioning of mitochondria, as well as in determining the branching pattern of mitochondria in striated muscle [15,34].
Little is known about the mitochondrial network structure associated with F-actin in response to mechanical loading in bone cells. Therefore, we aimed to investigate the regulation of bone cell mitochondrial structure and dynamics by mechanical loading. We focused on the movement (track speed and track displacement) and network structure (footprint, mean network branch, and mean branch length) of mitochondria in live pre- osteoblasts subjected to pulsating fluid flow (PFF) as well as on the movement of F-actin (top and side view). We analyzed the trajectory of mitochondria (quantification) and of F-actin (visual inspection) triggered by PFF in pre-osteoblasts.
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