Page 104 - Physico-Chemical Niche Conditions for Bone Cells

PFF affects pre-osteoblast behavior
Figure 5. Long-term down-stream impact of PFF on osteogenic differentiation of MC3T3-E1 pre- osteoblasts. ALP protein and collagen production were assessed after 21 days of culture, and ECM mineralization after 28 days of culture in osteogenic differentiation medium. After 1 h PFF, the medium was refreshed after 0, 1, 3, 6 h post-incubation without loading. (A) ALP staining of pre-osteoblasts. Scale bar=200 μm. (B) Quantitative colorimetric results of ALP staining. (C) Collagen staining of pre- osteoblasts. Scale bar=200 μm. (D) Quantitative colorimetric results of collagen production. (E) ECM mineralization. Scale bar=100 μm. (F) Quantitative colorimetric results of ECM mineralization. Values are mean ± SD. *Significant effect of PFF, p<0.05, n=3.
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