Page 101 - Physico-Chemical Niche Conditions for Bone Cells

formation by MC3T3-E1 pre-osteoblast without and with PFF. (H) Quantification of pseudopodia length in static control cells and PFF-treated cells. (I) Quantification of cell ratio (length/width) in static control cells and PFF-treated cells. (J) Histogram of frequency distribution of cell orientation of static control cells. (F) Histogram of frequency distribution of cell orientation of PFF-treated cells.
Figure 4. Short-term down-stream impact of PFF on MC3T3-E1 pre-osteoblast metabolic activity and gene expression. (A) Short-term down-stream impact of PFF on MC3T3-E1 pre-osteoblast metabolic activity at 0, 1, 3, and 6 h of post-incubation without mechanical loading. (B-H) Short-term down-stream impact of PFF on expression of osteogenic (Runx2, Ocn, Mepe, Dmp1, Col1a1), proliferation (Ki67), and angiogenic-related (Fgf2) genes. (B) Runx2, (C) Ocn, (D) Ki67, (E) Fgf2, (F) Mepe, (G) Dmp1, and (H) Col1a1 gene expression by MC3T3-E1 pre-osteoblasts treated with or without 1 h PFF at 0, 1, 3, and 6 h of post-incubation without mechanical loading. Values are normalized to Pbgd expression. Values are mean ± SD. *Significant effect of PFF, p<0.05, **p<0.01. n=3-6.
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