Page 101 - Effects of radiotherapy and hyperbaric oxygen therapy on oral microcirculation Renee Helmers

Late radiation-induced oral microvascular changes
undergone surgery in the HN region in <6 months (which both may influence vascularization), or had severe trismus preventing intraoral measurements. History of general health and past irradiation dose were obtained with patient approval. Dentate or edentulous status was noted. A group of healthy age- matched volunteers (ASA 1; including smokers, social drinkers, and 19<BMI<30) was included and used as a control group. To obtain an indication of systemic health and to exclude unknown cardiovascular disease, basic hemodynamic parameters (heart rate, systolic, diastolic, and mean arterial blood pressures) and body temperature were recorded for each control subject.
Microvascular imaging
Microcirculation measurements were performed using a CytoCam (CC)
Microscope System (Braedius Medical, Huizen, The Netherlands); details
on the CC instrument are described elsewhere.1,15 Using incident dark-field
illumination and CMOS sensor-based imaging technology, the commercially
available CC is a lightweight handheld imaging instrument that operates by epi-
illuminating a tissue of interest with pulsed (2 ms) 530 nm wavelength green
light through light-emitting diodes (LEDs) located around the tip of the probe 5 lens. As hemoglobin from erythrocytes absorb the green light, the remaining
light scatters into the surrounding tissue. Using a 4× objective lens system (237× onscreen magnification), the imaging sensor records and processes the relayed tissue light and produces a clear and high-resolution image (14 megapixel, 25 fps) of dark circulating erythrocytes in the lumen of blood vessels contrasted by a bright background in a field of view (FOV) equal to 1.55×1.16 mm (1.80 mm2). The CC is connected to a fanless medical grade panel PC (Braedius Medical, Huizen, The Netherlands) equipped with the CCTools software (CytoCamTools Camera Manager v1.7.12, Braedius Medical, Huizen, The Netherlands) for camera operation and video data processing.
Measurement procedures
Microcirculation measurements were performed in the same examination room kept at a constant temperature of 20±2°C. Participants were seated upright in a chair with their head resting against the wall to limit head movement. Prior to measurements the participants were requested to refrain from eating, drinking (other than water), and any oral hygiene practices for at least 30 min. Participants were asked to briefly rinse their mouth with lukewarm (~40°C) water since most HNCP have a dry mouth; the same procedure was performed
99