Page 72 - Tyrosine-Based Bioconjugations - Jorick Bruins
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Chapter 4
Figure 3. SDS-PAGE analysis of IEDDA reaction of Tras[KiH-HC]TCO with MeTz–TAMRA (lane 2), MeTz–IL-2 (lane 3) and MeTz–UCHT1 (lane 5), and Tras[KiH-HC]MeTz with BCN–lissamine (lane 8), TCO–IL-2 (lane 9), and TCO–UCHT1 (lane 11). Lanes M are loaded with protein ladders, lanes 1 and 7 contain the unmodified KiH antibodies.
Having successfully demonstrated the suitability of tetrazine ligation for the generation of [2]:1 antibody-protein conjugates, we also explored the inverse tetrazine ligation strategy. Thus, Tras[KiH-HC]MeTz was subjected to conjugation with either small-molecule fluorophore BCN–
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