Page 55 - Tyrosine-Based Bioconjugations - Jorick Bruins
P. 55
Abstract:
Reaction of cyclopropanated trans-cyclooctene (cpTCO) with in situ generated ortho-quinone is an efficient tool for bioorthogonal protein conjugation. The (4+2)-cycloaddition of cpTCO with ortho-quinone is significantly faster than its cyclooctyne counterpart (BCN). Orthogonal, tandem cpTCO–quinone and BCN–azide cycloadditions afforded a homogeneous, dual labelled antibody– drug conjugate.
This work was published as:
Jorick J. Bruins, Daniel Blanco-Ania, Vincent van der Doef, Floris L. van Delft and Bauke Albada
Chem. Commun., 2018, 54, 7338-7341
Reproduced by permission of The Royal Society of Chemistry
