Page 15 - Tyrosine-Based Bioconjugations - Jorick Bruins
P. 15
binding site for each epitope, i.e. a [1:1] format. Other variants of these heterodimeric antibodies include charge-pairing, where heterodimerization is promoted via negatively and positively charged residues, have been reviewed in length.15, 17
Figure 2. Schematic representations of (A) full size antibody with depicted termini, (B) the two permutations of scFv fragments, (C) tandem scFvs with two different scFvs, (D) bispecific antibody with C-terminally fused scFvs as a [2]:2 format, and (E) bispecific knob-in-hole antibody as a [1:1] format.
Higher order functionalities of antibody specificity exist. For example, having a knob-in-hole antibody with identical Fab fragments but two distinct fused scFv fragments would allow for a
General Introduction
13
1
