Page 133 - Tyrosine-Based Bioconjugations - Jorick Bruins
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Inducible, Selective Labelling of Proteins via Enzymatic Oxidation of Tyrosine
586 nm for Lissamine, i.e. sulphorhodamine B; Figure 4A). No labelling of the light chain is detected.
Figure 4. (A) Schematic representation of Tras[HC]G4Y before (i) and after (ii) labelling with BCN-Lissamine or (iii) labelling with cpTCO-Lissamine. (B) SDS-PAGE analysis and (C) HPLC analysis after the SPOCQ reaction performed according to protocol.i
Methods - HPLC Analysis
General HPLC (or LCMS) workup for SPOCQ performed on antibodies (See note 14):
i Bruins, J. J., Blanco-Ania, D., van der Doef, V., van Delft, F. L., and Albada, B., Orthogonal, dual protein labelling by tandem cycloaddition of strained alkenes and alkynes to ortho-quinones and azides. Chem. Commun. 2018, 54 (53), 7338-41. Reproduced by permission of The Royal Society of Chemistry.
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