Page 43 - Tyrosine-Based Bioconjugations - Jorick Bruins
P. 43
Abstract:
Genetically encoded tyrosine (Y-tag) can be utilized as a latent anchor for inducible and site- selective conjugation. Upon oxidation of tyrosine with mushroom tyrosinase, strain-promoted cycloaddition (SPOCQ) of the resulting 1,2-quinone with various bicyclo[6.1.0]nonyne (BCN) derivatives led to efficient conjugation. The method was applied for fluorophore labelling of laminarinase A and for the site-specific preparation of an antibody-drug conjugate.
This work was published as:
Reproduced by permission of The American Chemical Society
Jorick J. Bruins, Adrie H. Westphal, Bauke Albada, Koen Wagner, Lina Bartels, Hergen Spits, Willem J. H. van Berkel, and Floris L. van Delft
Bioconjugate Chem., 2017, 28 (4), 1189-1193
