Page 13 - Tyrosine-Based Bioconjugations - Jorick Bruins
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Figure 1. (A) Schematic representation of IgG1. Heavy chains are depicted in blue and the light chains in green. The interchain disulfide bonds are depicted in red. (B) Zoom of the hinge-region of IgG1. (C) Crystal structure of an IgG1 (PDB: 1igt9). (D) The most abundant10, 11 glycoforms for IgG1.i
1.1.1. Antibody digestion and fragmentation
For analytical purposes, digestion of antibodies via enzymatic, chemical or genetic means can be beneficial. Chemical digestion consists mostly of reducing the disulfide bonds by β- mercaptoethanol (BME), dithiothreitol (DTT), or tris(2-carboxyethyl)phosphine (TCEP), resulting
i The graphical representation of glycans are according to their nomenclature.10
General Introduction
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