Page 88 - Molecular features of low-grade developmental brain tumours

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Supplementary information
Supplementary Figure 1. Principle variance component analysis (PVCA) of all the methylation profiles of both SEGA and control samples. A PVCA was performed to quantify the contribution of various variables to the overall variance between the samples (SEGA n=42 and control tissue n=8). The major contributor to the overall variance was the diagnosis of the samples (SEGA or control). Other clinical data including TSC1/TSC2 mutation status, gender, localization of the SEGA, size of the tumour, epilepsy, age at seizure onset, seizure frequency, drug management at time of surgery (including treatment with mTORC1 inhibitors), tumour recurrence/regrowth and presence of other TSC related malformations as well as their various interactions contributed minimally to the overall variance seen amongst the samples. The residual is all variance that cannot be explained by the known factors.
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