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FIGURE 6. Selected kinome profiling-detected Shh-provoked signal transduction events and the role of Patched and Smoothened therein. Blue elements are confirmed, whereas gray elements showed a trend but did not reach Bonferoni-corrected statistical significance. The results reveal that the role of Patched dependent Smoothened- independent signal transduction is more prominent in transcription independent cellular effects of Hedgehog as previously thought.
Cellular kinase response to selective Smoothened activation
Subsequently we decided to investigate the effects of selective Smoothened activation in MEFs. To this end we employed purmorphamine, a purine derivative that directly targets Smoothened[45, 51] and employed the compound to challenge MEFs. The results are provided through Figure 4 and Supplementary table 1. We
Noncanonical Hedgehog signaling
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