Page 37 - Development of Functional Scaffolds for Bone Tissue Engineering Using 3D-Bioprinting of Cells and Biomaterials - Yasaman Zamani
P. 37

Laboratories Inc., Veenendaal, The Netherlands). Constructs were air-dried at room temperature for 24 h and weighed. Data were normalized to the weight of the dried constructs and expressed as absorbance/g of construct. Constructs were assayed in triplicate.
Statistical analysis
Data are expressed as mean ± standard deviation (SD). Differences in mean values were analyzed by one-way ANOVA with Tukey’s multiple comparison test using GraphPad Prism® 7.0 (GraphPad Software Inc., La Jolla, CA, USA). Differences were considered significant if p<0.05.
RESULTS
Optimum NaOH concentration for surface modification of 3D-printed PCL scaffolds
Proliferation of cells on PCL scaffolds treated with 1, 3, or 5 M NaOH was significantly increased compared to unmodified controls (Fig. 1a). After 7 days of culture, cell proliferation reached a maximum on PCL scaffold treated with 5 M NaOH, while proliferation still continued on scaffolds treated with 1 and 3 M NaOH (Fig. 1a).
Figure 1. Concentration-dependent effect of NaOH surface treatment of 3D-printed PCL scaffolds on MC3T3-E1 proliferation and collagenous matrix deposition after 8 days of culture. (a) Surface treatment of PCL scaffold with 1, 3, and 5 M NaOH significantly increased proliferation of cells after 8 days of culture. Cell number started to decrease on PCL scaffold treated with 5 M NaOH after 7 days of culture. (b) Collagenous matrix deposition by MC3T3-E1 on PCL scaffolds treated with 1, 3, or 5 M NaOH visualized by picrosirius red staining after 8 days of culture. More collagen (red) was observed on PCL scaffold treated with 3 M NaOH compared to scaffolds treated with 1 or 5 M NaOH. Scale bar, 500 μm. Black arrows, collagen.
 35



























































































   35   36   37   38   39