Page 38 - Cellular Imaging in Regenerative Medicine, Cancer and Osteoarthritis
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                                Chapter 2
2.2.4. Labeling efficiency in other cell types
Based on all findings, i.e. labeling efficiency in terms of percentage of cells labeled, label incorporation, label retention and retention of cell viability and cell function, we found the optimal labeling conditions for HUVECs to consist of a labeling dose of 12.5 mg/2 ml/9.5 cm2 and an incubation time of 24 h in the case of SPIO, and in the case of MPIO to consist of a labeling dose of 50 mg/2 ml/9.5 cm2 and an incubation time of 4 h.
A
CD
Figure 4 MR visibility of labeled cells. Representative images showing the high imaging sensitivity that can be reached for the various labeling conditions. A Composition of light microscopy images showing a small sample preparation (demarcated region of interest is approximately 1 mm by 1 mm) of (labeled) HUVECs in culture as used from MR imaging. B MR Image of a comparable region of interest of a sample containing unlabeled cells. C MR Image of a comparable region of interest of a sample containing cells labeled with SPIO at a dose of 12.5 mg/2ml/9.5 m2 for 24 hrs 5 days after labeling. D MR Image of a comparable region of interest of a sample containing cells labeled with MPIO at a dose of 25 mg/2ml/9.5 m2 for 4 hrs 5 days after labeling.
      B
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