Page 109 - Cellular Imaging in Regenerative Medicine, Cancer and Osteoarthritis
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                                Stabilized Sarabesin-3 for prostate imaging
 Figure 1 The concept of radio-guided surgery: A Radiopharmaceuticals directed at tumor cells are administered to the patient followed by accumulation of radioactivity in these cells over time. B Subsequently, full body nuclear imaging is performed, e.g. SPECT/MRI, to localize the tumor pre-operatively. C Using this information the patient is operated for removal of the tumor. Intra- operatively a handheld device is used to detect radioactivity derived from the tumor cells to guide the surgeon towards the tumor.
Figure 2 Structure of SB3.
total volume was added to 70 nmol of peptide in PBS, and the mixture was allowed to react for 5 min at room temperature. To exclude non-labeled iodine, a purification was performed by solid phase extraction using a J.T. Baker C18 cartridge followed by HPLC purification [34].
Internalization studies
Internalization with [111In]SB3 was performed in PC-3 cells, a commonly used human derived androgen independent GRPR-expressing PC cell line (AACR). The cells were seeded in six-well plates at a density of 0.6x106 cells per well and cultured overnight. On the day of the experiment cells were washed twice with 37°C PBS. Fresh internalization medium, RPMI + Glutamax with 20mM HEPES
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