Page 70 - The autoimmune hypothesis of narcolepsy and its unexplored clinical features M.S. Schinkelshoek

Chapter 4
Figure 4.2. Frequencies of major immune cell lineages are comparable between NT1 patients with recent disease onset and HLA- DQB1*06:02-matched healthy controls. Relative frequency of major immune cell lineages in samples from NT1 patients with recent disease onset (bright red), later disease onset (burgundy), HLA-DQB1*06:02-matched healthy controls (bright green) and non-HLA matched controls (dark green) are shown as a percentage of all CD45+ cells per group. Frequencies of none of the major immune cell lineages differed significantly between NT1 patients with recent disease onset compared to either of the other three participant groups.
HLA = human leucocyte antigen; NT1 = narcolepsy type 1; TCR = T cell receptor.
Figure 4.3. Repeated HSNE analyses lead to the identification of distinct memory CD4+ T cell populations. All landmarks are selected and embedded at the next, more detailed levels showing a finer granularity of structures. Each depicted level is the result of a new HSNE analysis on a single population delineated in the former level. This figure shows more detailed levels for the memory CD4+ T cell lineage. Unsupervised GMS clustering was used to identify phenotypically distinct immune cell clusters based on their density features. Black dots indicate the centroids of the identified clusters (as can be seen in the density map on the right).
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