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Figure 5. Differentiation of fetal neural stem cells (NSCs) after transfection with miR146a and miR147b mimics. NSCs were transfected with miRNA mimic (50 nM) for 24 hours, followed by 7 days of differentiation. A-L Confocal images of fixed and immunostained cells and phase con- trast pictures of live cells in culture. Cells were stained for βIII-tubulin, GFAP and counterstained with DAPI. The βIII-tubulin-positive cells and DAPI nuclei were quantified. M The βIII-tubulin/DAPI ratio was increased after transfection with miR146a or miR147b mimics. N and O Quantitative real-time PCR of target mRNA expression in NSC cultures 48 hours after initiation of differentia- tion. N NOTCH1 expression was decreased after transfection with miR146a or miR147b mimics. O miR147b mimic decreased the expression of JAK2. Scale bar in K and L: 50 μM. Experiments were performed in triplicate in cultures from three separate donors and data are mean ± SEM. *p<0.05, **p<0.01 compared to control, Mann Whitney U test.
































































































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