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Figure 3 In situ hybridization of miR146a in control, perituberal cortex, TSC cortical tuber and SEGA. A-B: miR146a, control grey (A) and white matter (B); miR146a was expressed in neurons (arrows in A), but was undetectable in glial cells. C: perituberal cortex (peri-ctx), showing low ex- pression of mi146a. D-G (cortical tuber), showing strong expression of miR146a within the tuber; arrows in E indicate dysmorphic neurons (high magnification, insert a); arrowheads in E and ar- rows in F indicate cells with glial morphology (high magnification, insert b in E); insert c in E shows expression in a dysmorphic neurons (NeuN positive, red); insert in F shows expression of miR146a in a GFAP positive cell (red). G: expression in giant cells; insert in G: expression of miR146a in pS6 positive cells (red). H: mi146a expression in subependymal giant cell astrocytoma (SEGA); insert a: expression of miR146 in GFAP positive tumor cells; insert b in H: expression of miR146a in pS6 positive cells (red). Scale bar in H: A-B: 80 μm. C-D: 160 μm E-H: 40 μm.