Page 52 - Strategies for non-invasive managementof high-grade cervical intraepithelial neoplasia - prognostic biomarkers and immunotherapy Margot Maria Koeneman

Chapter 2
50
Biomarker Biomarker group
Assumed role in natural history of CIN
Studied in histologically confirmed CIN?
No of studies on high-grade CIN (incl CIN 2)
Prospective study design?
Study size (n=)
Evaluation according to the PROBE-criteria*
Effect
Cell cycle regulatory factors
P53 Tumour suppressor.
CIN 2-3 CIN 2-3 CIN 1-2-3
2 (7, 78)
3 (6, 7, 78)
3 (76, 78, 79)
No, yes Yes, no, yes Yes
55, 28
1. +/-, + 2. +/-, +/- 3. +, +
4. -, -
Expression of p53 is consistently asso- ciated with disease regression.
pRb Tumour suppressor.
162, 55, 28
1. +, +/-, +
2. +/-, +/-, +/- 3. +, +, +
4. -, -, -
Expression of pRb is consistently associated with disease regression.
Ki67 Cell proliferation marker.
90, 28, 79
1. +, +, +
2. +/-, +/-, +/- 3.+,+,+ 4.-,-,-
Ki67 expression
is associated with progression of CIN 1-2, but not with progression of CIN 2-3 (small study)
Rb Tumour suppressor.
CIN 1-2 CIN 1-2-3
1 (76)
3 (7, 78, 82)
Yes
No, yes, yes
90
1.+ 2. +/- 3.+ 4.-
Expression of Rb
is associated with disease regression.
P16 Surrogate marker: no function in cellular
55, 28, 52
1. +/-, +, +
2. +/-, +/-, +/- 3.+,+,+ 4.-,-,-
Conflicting results: p-16 expression is associated with progression of CIN 2 in one study. Con- tradicting results
processes.
in two studies on CIN 2-3.
table continues