Page 168 - Physico-Chemical Niche Conditions for Bone Cells
P. 168

General discussion
improved bone regeneration and osteointegration of implants. Mesenchymal stem cells (MSCs) seeded on RGD-functionalized SLBs substrate for 10 days are more osteogenic, e.g. higher ALP activity [39]. This data is in line with the results of our study, but we did not investigate why and how RGD-functionalized SLBs affect osteogenic differentiation. Future studies will focus on the mechanism of osteogenic differentiation guided by RGD- functionalized SLBs.
Figure 1. Schematic diagram of osteoblast morphology and function affected by physical and chemical factors. (A) Osteoblast morphology before treatment with physical and/or chemical factors. (B) Osteoblast morphology after treatment with physical and/or chemical factors. (C) Changes in structure and function of an osteoblast treated by a physical factor in B. (D) Changes in structure and function of an osteoblast treated by a chemical factor in B.
In this thesis, we demonstrated that mechanically-loaded osteoblasts change their surface area and volume, as well as the spatial and temporal distribution of adhesion and cytoskeletal proteins (Fig. 1), through soluble factors (e.g. NO production, Fgf2 expression, and Wnt signaling). This may indicate that morphological changes in bone cells drive cell behavior and function, which might relate to diseases, e.g. osteoporosis. During estrogen deficiency, osteoporotic bone cells undergo similar or higher maximum strains than healthy
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