Page 121 - Physico-Chemical Niche Conditions for Bone Cells
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Parameter
Footprint
Mean network branch Mean branch length
Description Reference Total area of mitochondria in the image. [37]
The average number of branches per network. [37]
The average length of all branches. [37]
Chapter 5
 Figure 2. Mitochondrial video analysis using Image J software. (A) Procedure of mitochondrial movement analysis. (B) Procedure of mitochondrial network structure analysis.
Table 1 Definition of mitochondrial parameters
       were opened by Image J, transferred RGB color into 8-bit, and adjusted Brightness/Contrast using Auto click. Second, StuartLab was found in the list of Plugins (Fig. 2B).
Statistical analysis
The data of mitochondrial footprint, mean network branch, and mean branch length are presented as mean ± SD (3 cells from 1 glass slide). Data were statistically tested with One- way ANOVA combined with repeated measures ANOVA and Bonferroni’s multiple comparison test. Significant differences were considered at a p-value <0.05. Statistical analysis was performed using GraphPad Prism 9 (GraphPad Software, San Diego, CA, USA).
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