Page 110 - Physico-Chemical Niche Conditions for Bone Cells
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PFF affects pre-osteoblast behavior
might have affected osteogenic differentiation, e.g. via NO and/or Fgf2. On the other hand, soluble factors produced by the cells themselves as a result of PFF treatment might have affected osteogenic differentiation, e.g. via NO and/or Fgf2. A low concentration of NO induces cell proliferation, differentiation, early mineralization, and Fgf2 expression. Fgf2 produced by osteoblasts is stored in the ECM to control osteoblast differentiation [4]. The results of this study and our previous study [1] show that both NO and Fgf2 were up-regulated by PFF, which might affect the process of mineralization. The PFF-induced release of these soluble factors might have long-lasting effects on osteoblast differentiation.
In conclusion, our results indicate that mechanical stimulation in the form of PFF has immediate (within 5 sec) impact on fluid dynamics over the cells, and affects pre-osteoblast morphology in the short-term, which is associated with enhanced osteogenic differentiation and matrix production by these cells in the long-term. This indicates that a single bout of mechanical loading with indirect associated release of soluble factors stimulates osteoblast differentiation in the long-term. Future studies will unravel the underlying mechanism and provide new insights into whether, and which morphological changes as a result of PFF drive osteogenic differentiation.
FUNDING
This work was granted by the China Scholarship Council [CSC, No. 201608530156]. This work was also granted by Health-Holland (Project No. LSHM19016, “BB”).
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