Page 7 - Tyrosine-Based Bioconjugations - Jorick Bruins
P. 7

Table of Contents
Preface......................................................................................................................................................................... 6
Chapter 1....................................................................................................................................................................8
General introduction
Chapter 2.................................................................................................................................................................40
Inducible, Site-specific Protein Labelling by Tyrosine Oxidation–Strain-Promoted (4+2) Cycloaddition
Chapter 3.................................................................................................................................................................52 Orthogonal, Dual Protein Labeling by Tandem Cycloaddition of Strained Alkenes and
Alkynes to ortho-Quinones and Azides
Chapter 4.................................................................................................................................................................64
Highly Efficient Mono–functionalization of Knob-in-hole Antibodies with Strain-Promoted Click Chemistry
Chapter 5.................................................................................................................................................................78 Modular, Chemoenzymatic Preparation of Trifunctional Protein-antibody Conjugates with
Tailored Valencies
Chapter 6.................................................................................................................................................................96 Engineering of Tyrosine-Containing Peptide Loops Enables Non-terminal Protein Labeling
Chapter 7..............................................................................................................................................................110
Summary and General discussion
Appendix .............................................................................................................................................................. 122 Inducible, Selective Labelling of Proteins via Enzymatic Oxidation of Tyrosine
Acknowledgements........................................................................................................................................ 137 Publications........................................................................................................................................................ 140 Overview of completed training activities ....................................................................................... 141


















































































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