Page 92 - Helicobacter pylori and Gastric Cancer: From Tumor microenvironment to Immunotherapy
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 FIGURE 3. Effects of Hedgehog on the uptake of [3H]-sucrose and the influence of Smoothened inhibition thereon. Fibroblast cultures were grown in twenty-four-wells plates and incubated in a 1 ml containing 200 nCi of [3H]-sucrose in the presence or absence of either 1 μg/ml Shh and 10 μM cyclopamine. At the end of the experiment cells were extensively washed with ice-cold PBS and lysed in NP-40 for subsequent scintillation counting, As sucrose can only enter cells through fluid phase uptake, this provides a reliable measure of cellular endocytosis. We observe that Hedgehog stimulates fluid phase uptake and this effect does not require Smoothened as it is not sensitive to the Smoothened inhibitor cyclopamine
                                Chapter 4
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