Page 131 - Helicobacter pylori and Gastric Cancer: From Tumor microenvironment to Immunotherapy
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 encoded by the babA2 gene, binds to Lewis b antigens and ABO antigen[15, 16] Although three bab alleles have been identified (babA1, babA2, and babB), only the babA2 gene product is functionally active[17] Studies in Western populations have associated the presence of the babA2 gene with gastric cancer[16, 18]. The aim of this studies is to assess the genotype of H. pylori strains infecting patients with chronic gastritis through the evaluation of the prevalence of several genes coding for virulence factors.
Materials and Methods
This research was approved by the regional Medical Research Ethics Committee of Azad University of Medical Science on 19 Jul 2016 (No: 1311/28772) and all patients provided written informed consent for this research.
This study protocol conforms to the ethical guidelines of the 1975 Declaration of Helsinki as Reflected in a prior approval by the institution's human research committee.
Patients, Bacterial strains and cultivation
Our cohort consisted of 109 clinical isolates of H. pylori, 49 (45%) were from female and 60 (55%)
From male patients (sex ratio F/M: 0.82); the average age of the patients from which the isolates were obtained was 39 ±17 years. H. pylori was isolated employing gastric biopsies of patients presenting with gastritis, peptic ulcer or gastric cancer. Patients were not exposed to antimicrobial agents at least one week before endoscopy was performed. Following collection, gastric biopsy samples were homogenized and cultured onto Brucella agar supplanted with 5% sheep blood and antibiotics (Vancomycin, Amphotericin B and Trimethoprim). Culture plates were incubated at microaerophilic condition, 37 oC and high humidity for 5-7 days. Organisms were identified as H. pylori based on colony morphology, gram staining and positive oxidase, catalase and urease tests.
                                 H-pylori pathogenicity factors H.pylori pathogenic factors
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